 |
|
|
|
|
|
| In
vitro Electrophysiology |
|
| |
| |
| Isolated
neurons |
| • |
pharmacology
and physiology of ion channels and receptors from isolated
or cultured neurons using patch-clamp techniques |
| |
|
| Synaptic
plasticity |
| • |
research
into the effects of drugs on synaptic plasticity using brain
slice recording |
|
|
| Imaging
techniques |
| • |
fluorescence
imaging to study effects of drugs on changes in intracellular
calcium |
|
|
| Neuronal
transmission |
| • |
to measure
the effects of drugs on nerve conduction and neurotransmission |
|
| |
|
Electrophysiology plays a major role in discovering the functional
mechanisms involved in drug modulation of neurotransmission. Our
highly skilled team uses a variety of techniques, including whole-cell
patch-clamp recording from cultured or isolated neurons, to study
the properties of receptors and voltage-gated ion channels. Recordings
are also made from brain slices to study processes involved in synaptic
plasticity and to evaluate the role of native receptor subtypes
in neurotransmission.
Our techniques include: |
|
| |
|
| Recording
from engineered cell lines |
| •
|
patch-clamp
recording from cells transfected with human cDNA encoding
neurotransmitter receptors and ion channels |
|
|
| Xenopus
oocyte recording |
| • |
Using
voltage clamp recording on Xenopus oocytes injected with DNA
to study effects of mutations on receptor pharmacology and
function |
|
|
